. Ni tekinoroji yo muri tekinoroji yo muri tekinorobije ishingiye ku isesengura no gutondekanya selile imwe. Irashobora gupima byihuse no gushyira mubunini, imiterere yimbere, ADN, RNA, RNA, AntiUre, Antimine, antigens nibindi bikoresho byumubiri, kandi birashobora gushingira ku cyegeranyo cy'ibipimo.
Cytometer ihagaze ahanini ibice bitanu bikurikira:
1 Icyumba cya Flow na Fluidics
2 Laser Light Inkomoko na Sisitemu yo guhinduranya
Sisitemu 3 ya Optique
4 ibikoresho bya elegitoroniki, ububiko, kwerekana no gusesengura sisitemu
Sisitemu 5 yo gutondeka Akagari
Muri bo, laser sprition yoroheje yoroheje ya laser no mu bikorwa bya perm hakozwe hashyizweho igipimo nyamukuru cy'ibimenyetso bya fluorescence muri cytometry. Ubukana bwumucyo ushima no mugihe cyo guhura bufitanye isano nuburemere bwibimenyetso bya fluorescence. Laser ni isoko yumucyo ishobora gutanga uburebure bumwe, uburemere-bwinshi, hamwe no kumurika cyane. Nibintu byiza byoroheje byoroheje kugirango wuzuze ibyo bisabwa.
Hariho inzira ebyiri za silindrike hagati yinkomoko ya laser nicyumba cyumunsi. Iyi lens yibanda ku kibero cya laser hamwe nigice cyambukiranya cyasohotse muri laster Inkomoko ya Laser muri Belliptique yinjira mu kiti cya elliptique gifite igice gito cyambukiranya (22 μ5 × 66 × 66). Ingufu za Lalliptique muriyi SOLIPTICAL ikwirakwizwa ukurikije ikwirakwizwa risanzwe, kwemeza ko imbaraga zibangamira selile zinyura mukarere ka laser. Kurundi ruhande, sisitemu ya optique igizwe nimirongo myinshi yinzira, Pinholes, na Akayunguruzo, bishobora kugabanywa mumatsinda abiri: hejuru no kumanura icyumba cyurubuga.
Sisitemu ya Optique imbere yurugereko rwurubuga rugizwe nicyayi na pinhole. Imikorere nyamukuru ya lens na pinhole (mubisanzwe lens ebyiri hamwe na pinhole) nukwibanda kubice bya laser byasohotse kubice bya laster byasohotse hamwe ninkomoko ya elliptique ifite igice gito. Ibi bikwirakwiza ingufu za laser ukurikije ikwirakwizwa risanzwe, kwemeza ko bibangamiye insingali zinyuranyije na selile hakurya ya laser no kugabanya ubuvanze kumucyo.
Hariho ubwoko butatu bwingenzi bwungurura:
1.
2: Igice gito-cyuyunguruzo (SPF) - Gusa Emerera urumuri hamwe nuburebure buri munsi yagaciro kanini kunyuramo.
3: Akayunguruzo ka Bandpass (BPF) - Gusa bituma urumuri rwihariye rwuburebure kugirango unyure.
Guhuza Ibinyuranyo Birashobora kuyobora ibimenyetso bya fluorescence ku burebure butandukanye bwamafoto yumurongo wamafoto (PMTS). Kurugero, muyunguruzi kugirango ugaragaze ibirungo cyatsi (fitc) imbere ya PMT ni LPF550 na BPF525. Akayunguruzo gakoreshwa kugirango tumenye fluorescence ya orange-itukura (pe) imbere ya PMT ni LPF600 na BPF575. Akayunguruzo ka Sluorescence itukura (Cy5) imbere ya PMT ni LPF650 na BPF675.
Kuzenguruka cytometry ikoreshwa cyane mugutondekanya selire. Hamwe no gutera imbere ikoranabuhanga rya mudasobwa, iterambere ryumupfunyira hamwe nikoranabuhanga rya monoclonal, porogaramu ryayo muri biologiya, imiti, farumasi nibindi bice bigenda birushaho kwinshi. Izi porogaramu zirimo gusesengura selile, selile wanditse acoptose, kwandika selile, kwisuzumisha ibibyimba, isesengura ryibiyobyabwenge, nibindi
Igihe cyohereza: Sep-21-2023
